TOPIC：Microfluidics for cell manipulation and intracellular drug delivery
SPEAKER：Dr.Xiaoyun Ding, Mechanical Engineering Department, University of Colorado Boulder
TIME：March 30 (Thursday)10:00am
LOCATION：化学A楼528演讲厅(Room 528, Chemistry Building A)
INVITER：朱新远教授(Prof. Xinyuan Zhu )
Intracellular delivery has been a long-standing challenge in biological research and medical applications. In the past few decades, academic research on drug delivery has been focusing on developing delivery vehicles that pose immunogenicity risks and toxicity concerns (Nature Biotechnology, 2014, 32, 961). Limitations of current technologies motivate innovations of next generation precise delivery technologies that can deliver universal materials to key cell types and subcellular organelles with controllable dose. The transformative role of nanotechnology, Microfluidics and MEMS technology provide the opportunity for precise cell manipulation and controllable disruption of cell membrane, which are desired for the next generation of intracellular and subcellular delivery.
First, we developed a microfluidic platform called acoustic tweezers that can manipulate objects from nanometer to millimeter scale using sound waves. This platform has demonstrated many functions such as: 1) single cell/organism manipulation; 2) tunable cell/nanowire patterning; 3) multichannel cell/droplet sorting; and 4) high-efficiency circulating tumor cell separation.
Second, we demonstrated a vector-free microfluidic platform named disruption and field enhanced delivery. By integrating mechanical disruption and electric field into one single chip, we can directly deliver DNA into nucleus at high throughput, paving a new avenue for intracellular and nuclear delivery of hard-to-transfer cells such as immune cell and stem cell.
Dr. Xiaoyun Ding is currently a Tenure-Track Assistant Professor at the Department of Mechanical Engineering, University of Colorado Boulder. Before joining CU Boulder, he was a postdoctoral associate supervised by Profs. Klavs Jensen and Bob Langer at Massachusetts Institute of Technology. Dr. Ding received his B.S. from Fudan University (2006), M.S. from Chinese Academy of Science (2009), and Ph.D. from The Pennsylvania State University (2013). His current research interests are focused on biomedical devices and microfluidics for micro/nano manipulation and precise drug delivery. Dr. Ding has coauthored 23 journal publications (including Nature, Nature Biomedical Engineering, PNAS, Lab on Chip, Advanced Materials, and Analytical Chemistry etc.), 5 US patents, 15 international conference presentations, and received a number of prestigious awards such as the Baxter Young Investigator Award, The Best Student Paper Competition Award in IEEE, Rustum & Della Roy Innovation in Materials Research Award etc..